A copper photocathode based on bulk Cu(100) substrate with a 50 𝜇m-diameter nanostructured region was fabricated and integrated into an S-band radio-frequency gun. We report the procedure for production and preparation of high- quality copper cathodes with nanostructured area compatible with state-of-the-art S-band RF gun. Experimental results indicate absence of increased dark current up to 70 MV/m at the cathode.